A novel rare cell sorting microfluidic chip based on magnetic nanoparticle labels

Abstract Rare cell sorting with magnetic nanoparticles labels (MNPs) has been achieved by the aid of microfluidic chip, which offers an easy processing approach to sort rare cells from a trace original sample. However, its applications have limited in large part due presence some redundant MNPs sorted target affecting purity cells. This paper demonstrates novel cell-sorting chip based on polydimethylsiloxane (PDMS) and MNPs. The system combines mechanisms sorting, geometry selection manoeuvre achieve high efficient real-time monitoring. device array microcolums acting as micro-sieve is capable separating cells, background simultaneously while having outlet for subsequent studies. A finite element method simulation implemented quantify experimental conditions. Green fluorescent Hela (Hela-GFP) different concentrations are used experiments. results show ability this capture MNPs-labelled accuracy maximum recovery rate up 99.7%. proposed provides effective reliable tool biological clinical researches, paves way automated system.